Glycolysis begins with the conversion of glucose to glucose-6-phosphate. This is followed by the formation and splitting of fructose-1,6-bisphosphate into two molecules of triose phosphate. Glyceraldehyde-3-phosphate is then oxidized to 1,3-bisphosphoglyceric acid. The phosphate of phosphoglycerate molecules is relocated from the third to the second carbon to yield two molecules of 2-phosphoglycerate. Finally, phosphoenolpyruvate is converted to pyruvic acid with a generation of ATP.

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